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734,735 Senescent cells are characterized by cell cycle arrest, increased autophagy, and heightened apoptosis, along with markers such as p21 and the senescence-associated secretory phenotype (SASP)

In several experimental studies, different methods of extraction were used to identify the nutritional, medicinal, and therapeutic properties of fruiting bodies like mushrooms (14, 22)

These results show that although the expected therapeutic effect has not been achieved, AVV-based therapy has acceptable safety

Mitochondrial isolation was performed using the Mitochondria Isolation Kit for Cultured Cells based on the manufacturers manuals (Thermo Fisher Scientific)

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